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KMID : 0377519910160020119
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Chung-Ang Journal of Medicine
1991 Volume.16 No. 2 p.119 ~ p.130
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Effects of Ascorbate on Ca^(²+) -Activated K^(+) Current in the Gastric Circular Smooth Muscle Cells
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Abstract
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In order to observe the effect of ascorbate on the Ca´+-activated K+ current in pyloric circular muscle cells which plays a critical role in generation and maintenance of repolarization and automatism, apart of pyloric circular muscle tissue was excised out, digested in collagenase-containing Ca" -free physiological solution to get single circular muscle cells and the whole cell mode of patch´-clamp experiment was conducted.
Following results were obtained
1. Oscillatory outward current was reduced by ascorbate in case of high intrapipette Ca´ concentration in whole voltage range and was enhanced in case of low intrapipette Ca" concentration.
2. Oscillatory outward current was reduced in Ca"-free physiological solution with calcium channel blocker and not effected by ascorbate.
& There were no qualitative differences among the control and ascorbate-treated group in ef-
fects of pH, steady state inactivation and the remaining component of the outward current.
The above results could be concluded that ascorbate effects calcium-dependent component of the oscillatory outward current in the pyloric circular smooth muscle cells.
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